This panel tests major immune cell lineages, such as T lymphocytes, B lymphocytes, monocytes, dendritic cells (DCs), and Nature Killer cells (NKs). It is also able to identify subsets and cell status of lymphocytes including regulatory T cells, memory T cells, and helper T cells, T cell activation, and myeloid and plasmacytoid DCs.
In Vitro basophil activation is used to evaluate IgE-dependent allergic reactions from various allergens, such as food, drugs, venom, etc. It is a valuable tool to assist in diagnosing allergic diseases, predict and monitor clinical responses to immunotherapy.
1. Lymphocyte Core Panel
The Lymphocyte Core Panel consists of 8 markers, including a live/dead reagent, which allows multiple T cell subsets (e.g. helper, cytotoxic, NK-T cells, activated T cells) to be identified, in addition to B cell detection.
2. TBMNK Panel
The TBMNK panel is designed to identify human total CD3⁺ T cells, CD4⁺ and CD8⁺ T cell subsets, total CD19⁺ B cells, two NK cell subsets (CD56brightCD16⁻ and CD56dimCD16⁺), three monocyte subsets (CD14⁺CD16⁻ classical, CD14⁺CD16⁺ intermediate and CD14low/⁻CD16⁺ non classical).
3. CD4+ T cell subset Panel (12 colors)
This panel is used to identify subsets of T lymphocytes, T helper cells (Th1, Th2 and Th22), regulatory T cells (Tregs) and T follicular helper cells (Tfh).
4. Treg Panel
The Treg backbone panel is designed for the identification of major subsets of CD3⁺CD4⁺CD25⁺CD127 ⁻ Tregs. It enables the characterization of naïve and memory/effector Tregs, the latter being further subdivided in CD15s⁺ highly differentiated Tregs and CD161⁺ inflammatory cytokine-producing Tregs.
5. Activated T cell Panel
This panel include seven activation-induced markers (AIM) in either human CD4⁺ or CD8⁺ T cells, and it can be useful the characterization of rare antigen-specific T cells and the assessment of vaccine immunogenicity.
6. T cell senescence and exhaustion panel
This panel is used to identify major subsets of CD4⁺ or CD8⁺ T Cells, including naïve, stem cell memory, central memory, effector memory, terminally differentiated effector T cell, senescent and exhausted T cells.
7. T Cell Inhibitory Receptor Panel
This panel is used to measure the expression of six inhibitory receptors in CD4⁺ or CD8⁺ T cells and detailed assessment of multiple markers associated with T cell exhaustion. The presence of antibodies for the detection of differentiation markers further allows the assessment of these inhibitory receptor expression patterns within these subsets of T cells.
8. B cell subset panel
This panel is used to identify major subsets of B cells, including transitional, naïve, memory B cells (class-switched and non-class-switched) and antibody-secreting cells (ASCs)/plasma blasts.
9. NK cell panel
The NK cell activation and inhibitory receptor panel is designed for the identification of distinct subsets of NK cells based on the expression of four differentiation markers, five activating receptors and six inhibitory receptors.
10. Dendritic cell panel
This panel is designed for the identification of six major DC subsets including cDC1, cDC2, CD123⁺Axl⁺ DCs, CD11c⁺Axl⁺ DCs, pDCs and CD16⁺ DCs1,2.
